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tmb  (Thermo Fisher)


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    Structured Review

    Thermo Fisher tmb
    Tmb, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/3+3+5+5+tetramethylbenzidine+tmb/3%2C3'%2C5%2C5'-Tetramethylbenzidine+soln%2E%2C+Ready-to-Use%2C+high+sensitivity/pm42330958-321-4-11
    Average 95 stars, based on 1 article reviews
    tmb - by Bioz Stars, 2026-09
    95/100 stars

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    Related Articles

    other:

    Article Title: Expression of bluetongue virus core proteins VP2 and VP7 by bovine herpesvirus-4 confers protection against virulent challenge in a mouse model
    Article Snippet: After several washes, the plates were developed with 3,3′,5,5′-tetramethylbenzidine (TMB) (Thermo Fisher Scientific, Waltham, MA, USA).

    Article Title: Combined pH-shifting and glycosylation treatment enhances functionality and mitigates allergenicity of β-lactoglobulin.
    Article Snippet: As a major milk allergen with limited functionality, β-lactoglobulin (β-Lg) requires effective modification strategies to enhance its applicability.. This study investigated the synergistic effects of pH-shifting pretreatment and glycation on the structure, functional properties, and allergenic potential of β-Lg.. The protein was first treated under strongly acidic or basic conditions to induce conformational rearrangements, followed by glycation with galacto-oligosaccharides (GOS) or xylo-oligosaccharides (XOS).

    Article Title: Bio-inspired branched Au–Cu nanoalloys for achieving synergistic dual-pathway peroxidase-like activity
    Article Snippet: 3,3′,5,5′-Tetramethylbenzidine (TMB) was also purchased from Alfa Aesar.

    Article Title: Click chemistry-based bioconjugates for direct immunochemical analysis of the mycotoxin citrinin.
    Article Snippet: Acros Organics 3,3′,5,5′-tetramethylbenzidine (TMB) was provided by Thermo Fisher Scientific (Waltham, MA, USA).

    Food & Beverages:

    Article Title: Hydrogen-bonded porphyrin frameworks hosting single-atom iron centers as robust peroxidase mimics for food antioxidant quantification
    Article Snippet: Rapid and accurate antioxidant detection is essential for assessing food quality, yet current total antioxidant capacity (TAC) assays remain constrained by limited sensitivity and susceptibility to matrix interference.. Herein, we report a hydrogen-bonded organic framework nanozyme featuring isolated iron sites (HOF-FeTCPP) for colorimetric detection of food TAC.. HOF-FeTCPP is assembled from iron-porphyrin building blocks into an ordered crystalline framework, wherein dispersed Fe(III) centers confined within the HOF matrix generate an enzyme-mimetic microenvironment that markedly enhances catalytic efficiency.

    Enzyme-linked Immunosorbent Assay:

    Article Title: 3D printing monolithic, multifunctional polymer acoustofluidic devices with tunable mixing and particle focusing
    Article Snippet: .. Subsequently, 150 μL of 3,3′,5,5′-tetramethylbenzidine (TMB) (1-StepTM TMB ELISA Substrate Solution, Thermo ScientificTM) was added to the magnetically separated complex, resulting in the development of a blue color. .. Afterward, 200 μL of 2.5 M sulfuric acid (ACS reagent, 95.0–98.0%, Sigma-Aldrich) was added as a stop solution, causing the solution to turn yellow.

    Incubation:

    Article Title: A STAT1-Knockout Mouse Model for Chapare Virus Infection and Pathogenesis
    Article Snippet: Plates were washed 3× with PBST and then incubated with either horseradish peroxidase (HRP)-conjugated goat anti-mouse IgG (Jackson ImmunoResearch Laboratories, West Grove, PA, USA) or HRP-conjugated goat anti-mouse IgM (Jackson ImmunoResearch Laboratories, West Grove, PA, USA) for one hour at ambient temperature. .. Plates were washed 3× with PBST and incubated with the substrate 3,3′,5,5′′-Tetramethylbenzidine (TMB) (ThermoFisher Scientific, Waltham, MA, USA) for 30 min at ambient temperature. .. Plates were read at 450 nm (SpectroMax M5; Molecular Devices, San Jose, CA, USA) after the addition of stop solution (0.16M sulfuric acid).

    Article Title: A STAT1-Knockout Mouse Model for Chapare Virus Infection and Pathogenesis
    Article Snippet: Plates were washed 3× with PBST and then incubated with either horseradish peroxidase (HRP)-conjugated goat anti-mouse IgG (Jackson ImmunoResearch Laboratories, West Grove, PA, USA) or HRP-conjugated goat anti-mouse IgM (Jackson ImmunoResearch Laboratories, West Grove, PA, USA) for one hour at ambient temperature. .. Plates were washed 3× with PBST and incubated with the substrate 3,3′,5,5′′-Tetramethylbenzidine (TMB) (ThermoFisher Scientific, Waltham, MA, USA) for 30 min at ambient temperature. .. Plates were read at 450 nm (SpectroMax M5; Molecular Devices, San Jose, CA, USA) after the addition of stop solution (0.16M sulfuric acid).



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